Archive

  • Visit JGI.DOE.GOV
News & Publications
Home › Publications › Functional analysis of Salix purpurea genes support roles for ARR17 and GATA15 as master regulators of sex determination

Functional analysis of Salix purpurea genes support roles for ARR17 and GATA15 as master regulators of sex determination

Published in:

Plant Direct 7(11) , e3546 ( 2023)

Author(s):

Hyden, Brennan, Carper, Dana L., Abraham, Paul E., Yuan, Guoliang, Yao, Tao, Baumgart, Leo, Zhang, Yu, Chen, Cindy, O'Malley, Ronan, Chen, Jin‐Gui, Yang, Xiaohan, Hettich, Robert L., Tuskan, Gerald A., Smart, Lawrence B.

DOI:

10.1002/pld3.546

Abstract:

The Salicaceae family is of growing interest in the study of dioecy in plants because the sex determination region (SDR) has been shown to be highly dynamic, with differing locations and heterogametic systems between species. Without the ability to transform and regenerate Salix in tissue culture, previous studies investigating the mechanisms regulating sex in the genus Salix have been limited to genome resequencing and differential gene expression, which are mostly descriptive in nature, and functional validation of candidate sex determination genes has not yet been conducted. Here, we used Arabidopsis to functionally characterize a suite of previously identified candidate genes involved in sex determination and sex dimorphism in the bioenergy shrub willow Salix purpurea. Six candidate master regulator genes for sex determination were heterologously expressed in Arabidopsis, followed by floral proteome analysis. In addition, 11 transcription factors with predicted roles in mediating sex dimorphism downstream of the SDR were tested using DAP-Seq in both male and female S. purpurea DNA. The results of this study provide further evidence to support models for the roles of ARR17 and GATA15 as master regulator genes of sex determination in S. purpurea, contributing to a regulatory system that is notably different from that of its sister genus Populus. Evidence was also obtained for the roles of two transcription factors, an AP2/ERF family gene and a homeodomain-like transcription factor, in downstream regulation of sex dimorphism.

View Publication

Share this:

  • Click to share on Facebook (Opens in new window)
  • Click to share on LinkedIn (Opens in new window)
  • Click to share on Pinterest (Opens in new window)
  • Click to share on Twitter (Opens in new window)
  • Click to print (Opens in new window)
  • JGI.DOE.GOV
  • Disclaimer
  • Accessibility / Section 508
Lawrence Berkeley National Lab Biosciences Area
A project of the US Department of Energy, Office of Science

JGI is a DOE Office of Science User Facility managed by Lawrence Berkeley National Laboratory

© 1997-2025 The Regents of the University of California