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April 14, 2020

JGI Earth Month: Kjiersten Fagnan Reflects on Shelter-in-Place Effects

Fagnan JGI Earth MonthIn this brief captioned video, JGI Chief Informatics Officer Kjiersten Fagnan reflects on the personal and environmental impacts of the shelter-in-place order as part of JGI Earth Month. [Read More]

April 14, 2020

Genome Insider Episode 2: Role of Viruses in Releasing Greenhouse Gases? (2/2)

Logo of Genome Insider, podcast of the Joint Genome InstituteGenome Insider is available on Apple Podcasts, Google Play, Spotify, iHeart Radio, and TuneIn Radio. Subscribe today! The Genome Insider podcast presents research by Gary Trubl, a virologist at Lawrence Livermore National Lab. He’s using bioinformatics and isotopes to track how viruses in the thawing arctic influence the flow of soil carbon. JGI is a user facility of the Department of Energy (DOE) Office of Science and located at the Lawrence Berkeley National Laboratory in Berkeley, CA. [Read More]

April 8, 2020

How Filamentous Fungi Sense Food

The filamentous fungus Neurospora crassa eating plant biomass. (Vincent Wu)A team led by researchers at the University of California, Berkeley used a multi-omics approach to reconstruct and model gene regulatory pathways used by the filamentous fungus Neurospora crassa, and to identify and decide on the order in which this fungus breaks down plant cell wall materials including lignin, cellulose and hemicellulose. [Read More]

March 10, 2020

Genome Insider Episode 1: Role of Viruses in Releasing Greenhouse Gases? (1/2)

Logo of Genome Insider, podcast of the Joint Genome InstituteGenome Insider is available on Apple Podcasts, Google Play, Spotify, iHeart Radio , and TuneIn Radio. Subscribe today! The Genome Insider podcast presents research by Gary Trubl, a virologist at Lawrence Livermore National Lab. He’s using bioinformatics and isotopes to track how viruses in the thawing arctic influence the flow of soil carbon. JGI is a user facility of the Department of Energy (DOE) Office of Science and located at the Lawrence Berkeley National Laboratory in Berkeley, CA. [Read More]

February 21, 2020

Tanja Woyke Elected to American Academy of Microbiology

Tanja Woyke, PhD Microbial Genomics Program Lead, DOE Joint Genome InstituteJGI’s Tanja Woyke has been elected to the American Academy of Microbiology, joining 67 other new Fellows in the Class of 2020. Fellows are selected based on their records of scientific achievement and original contributions that have advanced microbiology. [Read More]

February 10, 2020

Viruses Reprogram Cells into Different Virocells

How a cell behaves as virocell largely depends on the infecting virus and the genomic similarity between host and virus. Pseudoalteromonas was infected with two unrelated viruses: siphovirus PSA-HS2 and podovirus PSA-HP1. The infections transformed the same bacterial host into two very different virocells, HS2-virocell and HP1-virocell. The HS2 siphovirus genome was much more similar to the host than the genome of HP1 podovirus and had better access to recycle existing host resources. In contrast, the HP1 podovirus needed to work harder at obtaining the resources needed for infection, and reprogrammed multiple host metabolisms. HS2 virocells had a comparatively higher fitness than HP1 virocells. (Figure by Cristina Howard-Varona)If it looks like a duck and quacks like a duck, so the adage goes, it must be a duck. But if the duck gets infected by a virus so that it no longer looks or quacks like one, is it still a duck? For a team led by researchers from The Ohio State University and the University of Michigan studying how virus infections cause significant metabolic changes in marine microbes, the answer is no. They refer to the infected microbial cells as virocells, a change in name which reflects the metabolic changes they’ve undergone. [Read More]

February 4, 2020

JGI Scientists Pen Genome Watch Articles

For the May 2019 Genome Watch article by Tanja Woyke. (Credit: Philip Patenall/Springer Nature Limited)JGI researchers are sharing their expertise in environmental genomics by writing for the column Genome Watch in Nature Reviews Microbiology. In 2018. Tanja Woyke, who leads the Microbial Program at the JGI, received a message from Andrea Du Toit, senior editor for Nature Reviews Microbiology, with an unusual opportunity: would JGI researchers consider regularly writing for the magazine’s column Genome Watch? [Read More]

January 22, 2020

Here, There and Everywhere: Large and Giant Viruses Abound Globally

Art illustration capturing giant virus genomic diversity. (Zosia Rostomian/Berkeley Lab)JGI-led team significantly expands the global diversity of large and giant viruses. While the microbes in a single drop of water could outnumber a small city’s population, the number of viruses in the same drop—the vast majority not harmful to humans could be even larger. Viruses infect bacteria, archaea and eukaryotes, and they range in… [Read More]

January 21, 2020

Inspiring STEM Careers Through a Hands-on Everglades Microbiome Study

Students in the 2018 Boca Raton Community High School A-Level Advanced International Certificate of Education (AICE) Biology class collected samples from the Arthur R. Marshall Loxahatchee National Wildlife Refuge for the pilot project between the class and the JGI. (Image courtesy of Jon Benskin)Journal publication caps JGI pilot project with fledgling Florida high school scientists. The Florida Everglades evokes images of fanboats skimming over swamps, while alligators peer through the waters and clouds of insects hover just above. Described as a “river of grass” that stretches some 580,000 square miles across southern Florida, they encompass a wide range… [Read More]

December 23, 2019

Expanding Virophage Diversity

Virophage discovery pipeline. (A) MCP amino acid sequences from reference isolated genomes and published metagenomic contigs were queried against the IMG/VR database with stringent e value cutoffs. All homologous sequences detected were then clustered together to build four independent MCP profiles. (B) The resulting four MCP models were used to recruit additional homologous sequences from the entire IMG/M system. All new sequences were clustered, and models were built creating a final set of 15 unique MCP HMMs. (C) These 15 unique MCP HMMs were then used to search two different databases for homologous sequences: the IMG/M system and a custom assembled human gut database containing 3771 samples from NCBI’s Sequence Read Archive (SRA). (D) The resulting set of 28,294 non-redundant (NR) sequences with stringent e value cutoffs was filtered by size and e by the presence of the four core virophage genes (high-quality genomes; HQ virophages). Finally, completeness of novel metagenomic virophage genomes wsa predicted based on circularity or presence of inverted terminal repeats (ITR). (Figure from Paez-Espino et al. Microbiome (2019) 7:157 https://doi.org/10.1186/s40168-019-0768-5)Virophages are small viruses with double-stranded DNA genomes that co-infect eukaryotic cells along with giant viruses. Almost all known virophage genomes share only four genes in common: major and minor capsid proteins (MCP and mCP, respectively), ATPase involved in DNA packaging, and PRO, a cysteine protease involved in capsid maturation. Recently reported in Microbiome, researchers… [Read More]
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