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October 8, 2018

Expanding Fungal Diversity, One Cell at a Time

Blyttiomyces helicus on spruce pollen grain. (Joyce Longcore)Pilot study demonstrates single cell genomics approach for fungal genomic diversity. They can be found on forest floors, swamps and in houses, ranging in size from smaller than the period on your smartphone’s keyboard to stretching over several city blocks. More than a million species of fungi are estimated to live on this planet, but… [Read More]

October 3, 2018

Preparing for a Sequence Data Deluge

At the Heradura Playa in Atacama Desert of Chile. Alessandro Airo's team is interested in the Atacama's microbial communities. (Courtesy of A. Airo)Accepted 2019 JGI Community Science Program proposals requested terabases of sequence data. Over the summer, the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility, set an “institutional best” record by sequencing 20.4 trillion bases (Terabases or Tb) in a single month in Walnut Creek, Calif. Given the… [Read More]

June 26, 2018

Reproducibility Matters in Large-Scale Maize Study

In the PNAS study, heritable microbes associated with maize roots were identified through a large scale, multi-year, and multi-site field study.Large scale study identifies core microbial community for maize rhizosphere. A plant’s health is affected not only by conditions such as water and temperature, but by the microorganisms that live around its roots. The rhizosphere microbiome, as this microbial community is known, regulates nutrient availability to the plant from the soil, and can impact plant… [Read More]

April 3, 2018

Freedom and Flexibility: Thinking Outside the Cell for Functional Genomics

The cell-free systems approach outlined in the proposal starts by lysing pre-optimized cells from selected strains, and then working with the lysates from these strains to express genes and pathways of interest in cell-free platforms (CFPS) which can be mixed in cocktails of varying ratios for easily and rapidly characterizing novel and improved pathways, speeding up the “build” and “test” portion of the design-build-test (DBT) cycle. (Diagram: Mike Jewett)Latest JGI-Funded Technology Development Proposal Focuses on Cell-Free Systems Over the past two decades, the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility at Lawrence Berkeley National Laboratory, has transitioned from a high-throughput genome sequencing center to a national user facility that provides researchers around the world… [Read More]

March 19, 2018

A Reference Catalog for the Rumen Microbiome

Depiction of rumen microbiome. (Ella Maru studio, http://www.scientific-illustrations.com/)Cultivation and sequencing effort targets economically and environmentally relevant microbes. The digestive tracts of ruminant (cud-chewing) animals such as cattle, sheep, and goats convert lignocellulosic plant matter to short-chain fatty acids used for nourishment with unparalleled efficiency, thanks to the activity of symbiotic microbes in the rumen. Rumen microbes play a vital role in allowing… [Read More]

January 29, 2018

Tracking Microbial Diversity Through the Terrestrial Subsurface

Metabolic functions of microbial communities vary during a geyser eruption. Deep underground, the earth beneath our feet is teeming with microbial life, the majority of which has yet to be characterized. Cut off from sunlight, these enigmatic organisms must obtain life-sustaining energy and carbon, which all living cells need, through other means. A pressing question… [Read More]

January 11, 2018

All in the Family: Focused Genomic Comparisons

Colonies of Aspergillus: A. campestris; A. ochraceoroseus; and, A.steynii. These 3 species were among those whose genomes were sequenced in the study. (Kirstine Ellen Lyhne, DTU)Genus-wide Aspergillus project highlights new functional genome annotation methods. Found in microbial communities around the world, Aspergillus fungi are pathogens, decomposers, and important sources of biotechnologically-important enzymes. Each Aspergillus species is known to contain more than 250 carbohydrate active enzymes (CAzymes), which break down plant cell walls and are of interest to Department of Energy… [Read More]

December 20, 2017

When One Reference Genome is Not Enough

A single reference genome is not enough to harness the full genetic variation of a species so pan-genomes of crops would be extremely useful. The phenotypic diversity of Brachypodium plants is shown here. (John Vogel)Much of the research in the field of plant functional genomics to date has relied on approaches based on single reference genomes. But by itself, a single reference genome does not capture the full genetic variability of a species. A pan-genome, the non-redundant union of all the sets of genes found in individuals of a… [Read More]

December 18, 2017

A Functional Genomics Database for Plant Microbiome Studies

Phylogenetic tree of 3,837 high quality and non-redundant bacterial genomes. Outer ring denotes the taxonomic group, central ring denotes the isolation source, and inner ring denotes the root-associated genomes within plant-associated genomes. Taxon names are color-coded based on phylum: green – Proteobacteria, red – Firmicutes, blue – Bacteroidetes, purple - Actinobacteria. (Asaf Levy)Catalog of candidate genes involved in plant-microbe relationships. As the global population rises, estimated to hit nearly 10 billion by 2050, so does the need to boost crop yields and produce enough plant material for both food and sustainable alternative fuels. To help improve crop breeding strategies and overcome challenges such as making plants more… [Read More]

October 30, 2017

White Rot Fungi’s Size Explained by Breadth of Gene Families Involved

Clusters of fruiting bodies emerge on and around trees in Armillaria-infected areas in the fall. (Virág Tomity)Comparative genomics involving humongous fungus helps explain evolution of Armillaria Among the contenders for the world’s largest living organism is something usually considered much smaller than a blue whale, or a towering sequoia. This particular organism is so big, one needs an aerial map to grasp its size, and even then it’s not completely visible… [Read More]
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