Comparative genomics method to tag novel nitrogen-fixation genes
Lessons from the adaptive strategies of a fungal pathogen
by ressaure Coniferous forests in Europe and North America have suffered several hundred million dollars in damages annually from the fungal pathogen Heterobasidion annosum. Aside from the economic losses, scientists are concerned by the white rot fungus that not only breaks down the wood for nutrients, but also releases the carbon dioxide trapped in the… [Read More]
Foxtail Millet Genome an Improved Reference for Switchgrass
The DOE is interested in switchgrass as a prospective biofuels feedstock, but its genome is complicated because it has multiple copies of its chromosomes. As the world leader in sequencing plants and other organisms for their relevance to DOE missions, the JGI has sequenced switchgrass and several other plants that are candidate plant feedstocks; other… [Read More]
Microbes as the unknown variable in thawing permafrost
Overcoming short-read genome sequence assembly challenges
The genome assembly challenges posed by short sequence reads from sequencing platforms such as 454/Roche and Illumina are well-documented; the lack of reference genome data can hinder attempts to put together the myriad of short DNA sequences. Researchers from the Georgia Institute of Technology and the DOE JGI wanted to determine the impact of short… [Read More]
An alternative surrogate for gene discovery in switchgrass
Selected for the DOE JGI 2007 Community Sequencing Program because of traits such as high yield, perennial growth and low nutrient requirements, switchgrass is a promising candidate bioenergy crop that could be used to develop cellulosic biofuels. One of the challenges with sequencing switchgrass is the fact that it is a polyploid, containing multiple, full… [Read More]
Effects of symbiosis on a fungal transcriptome
The soils of the boreal forests in the northern latitudes are estimated to store more than three times the amount of carbon contained in the atmosphere or in plant life on land. Recent studies suggest the relationship between fungi on the forest floor, plants and the microbial communities at the plant roots (or rhizosphere) plays… [Read More]
Analysis of a TCE-degrading metagenome
Searching for the basis of ligninolytic selectivity
Many fungal genome projects being carried out at the DOE JGI focus on understanding how enzymes can break down cellulose and lignin, the two most abundant biopolymers on Earth, in order to harness these capabilities for industrial applications such as biofuels production. Scanning electron micrograph of Ceriporiopsissubvermispora mycelium on wood. (Robert Blanchette, University of Minnesota)… [Read More]