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April 8, 2020

How Filamentous Fungi Sense Food

The filamentous fungus Neurospora crassa eating plant biomass. (Vincent Wu)A team led by researchers at the University of California, Berkeley used a multi-omics approach to reconstruct and model gene regulatory pathways used by the filamentous fungus Neurospora crassa, and to identify and decide on the order in which this fungus breaks down plant cell wall materials including lignin, cellulose and hemicellulose. [Read More]

February 10, 2020

Viruses Reprogram Cells into Different Virocells

How a cell behaves as virocell largely depends on the infecting virus and the genomic similarity between host and virus. Pseudoalteromonas was infected with two unrelated viruses: siphovirus PSA-HS2 and podovirus PSA-HP1. The infections transformed the same bacterial host into two very different virocells, HS2-virocell and HP1-virocell. The HS2 siphovirus genome was much more similar to the host than the genome of HP1 podovirus and had better access to recycle existing host resources. In contrast, the HP1 podovirus needed to work harder at obtaining the resources needed for infection, and reprogrammed multiple host metabolisms. HS2 virocells had a comparatively higher fitness than HP1 virocells. (Figure by Cristina Howard-Varona)If it looks like a duck and quacks like a duck, so the adage goes, it must be a duck. But if the duck gets infected by a virus so that it no longer looks or quacks like one, is it still a duck? For a team led by researchers from The Ohio State University and the University of Michigan studying how virus infections cause significant metabolic changes in marine microbes, the answer is no. They refer to the infected microbial cells as virocells, a change in name which reflects the metabolic changes they’ve undergone. [Read More]

December 20, 2019

Fishing for Novel Cellulose Degraders

Graphical representation of the cellulose hook approach. (Devin Doud)A “bait and hook” single-cell genomic approach to bioprospecting. The Science One of the most vital pieces of equipment for fly fishing is a boxful of lures. Designed with feathers or wires to mimic an insect or a particular movement, each of these lures are the bait designed to attract specific catches. A similar technique… [Read More]

December 2, 2019

Dealing with Drought: Uncovering Sorghum’s Secrets

Sorghum variety BTx642 grown in Central Valley at temperatures around 100 degrees for 65 days without water. It is still green and filling grain to almost the same extent as plants that were watered weekly. (Jeffrey Dahlberg, UC ANR Agricultural Research and Extension Center)Over 40 percent of the cereal crop’s genes respond to drought stress. The Science Fields of drooping stalks and cracked earth are becoming common images in many regions due to more extreme weather events such as heat waves, droughts and floods. The planet’s resources are being stretched by a growing human population and increasing demand… [Read More]

November 20, 2019

Making a Lichen Together

The lichen Gray’s Cup (Cladonia grayi), with its namesake goblet structures. (Thomas Barlow)For the first time, a team analyzes the transcriptomes of a lichen fungus and alga to understand their partnership more clearly. The Science The humble lichen is a superorganism: one being that is actually comprised of two (or more) participants. One is a fungus (usually belonging to the ascomycetes, one of the two main branches… [Read More]

August 20, 2019

Fungus Fuels Tree Growth

Poplar cuttings inoculated with M. elongata strain PM193 (far right) grow larger in 30 percent forest soil / 70 percent sand than without PM193 (middle). On the left are controls grown in sterile sand. (Chih-Ming Hsu)A better understanding of how poplar responds to endophyte associations with endophytes enables scientists to fine-tune their engineering efforts. [Read More]

July 15, 2019

Better Genome Editing for Bioenergy

The lipid producing yeast, Yarrowia lipolytica, examined with light microscopy (left) and fluorescence microscopy (right), after being stained with Nile Red to visualize the lipid droplets inside (shown here in white). (Courtesy of Hal Alper)A team has optimized a crucial part of CRISPR-Cas9 technology to enable improvements in microbial oil production. The Science CRISPR-Cas9 is a powerful, high-throughput gene-editing tool that can help scientists engineer organisms for bioenergy applications. Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective…. [Read More]

July 10, 2019

Cultivating Symbiotic Antarctic Microbes

FISH of Nha-C enrichment with Hrr. lacusprofundi ACAM34-hmgA. Fluorescence micrograph shows individual Nha-C cells amongst Hrr. lacusprofundi cells. Nha-C cells labelled with a Cy5 (red fluorescence) conjugated probe; Hrr. lacusprofundi cells labelled with a Cy3 (yellow fluorescence, recolored to green to improve contrast) probe; all nucleic-acid containing cells stained with DAPI (blue fluorescence). Composite image of all three filters. Scale bars represent 2 µm. (Josh Hamm, UNSW)Nanohaloarchaeota cultures reveal they are symbionts and not free-living organisms. The Science Researchers employed multiple microbiology and ‘omics techniques to experimentally determine that Nanohaloarchaeota are symbionts, rather than free-living organisms as had been originally thought. The Impact The Antarctic lakes are a “treasure trove” of unknown microbes that play critical roles in environmental processes (related… [Read More]

June 11, 2019

Developing Switchgrass for Biomass Production

Left to Right: Jerry Jenkins, JGI Plant Program head Jeremy Schmutz, Adam Healey and study senior author Tom Juenger of UT-Austin.Switchgrass community gardens help distinguish genetic bases of fitness traits from climactic influence. The Science To better understand the genetic basis of local adaptation, researchers established community gardens of switchgrass plants in 10 different field sites on a north-south gradient across the United States. Hundreds of the switchgrass plants in these gardens are clonally propagated… [Read More]

February 25, 2019

Evolution of a Fungal Gene Expression Regulator

Parasitella parasitica (ZyGoLife Research Consortium on Flickr, CC BY-SA 2.0)5mC is an important piece of how many organisms regulate their genomes, but it is not well understood in fungi. Researchers reported on the largest analysis of 5mC distribution across the fungal tree of life to date, involving more than 500 species of fungi. [Read More]
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