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July 5, 2017

Mutant Rice Database for Bioenergy Research

Genome-wide distribution of fast neutron-induced mutations in the Kitaake rice mutant population. (Guotian Li and Rashmi Jain)Genome-wide rice studies yield first major, large-scale collection of mutations for grass models. The Science Fast-neutron irradiation, exposure to high energy neutrons, induces a wide variety of mutations by making changes in DNA. Using this approach, rice researchers were able to create the first major, large-scale collection of mutations for grass models. Resequencing the 1,504… [Read More]

June 30, 2017

Lessons from Simulating A Deep Ocean Oil Spill

The oil slick in the Gulf of Mexico from the Deepwater Horizon oil spill as seen on April 29, 2010. (NASA Earth Observatory image created by Jesse Allen, using data provided courtesy of the University of Wisconsin’s Space Science and Engineering Center MODIS Direct Broadcast system)Metagenome pipeline helps researchers resolve near-complete microbial genomes involved in Deepwater Horizon oil spill. The Science The 2010 Deepwater Horizon oil spill released 4.1 million barrels of oil into the Gulf of Mexico and was the first major release of oil and natural gases into the deep ocean (1,500 meters). Due to the depth of… [Read More]

April 28, 2017

A Gene that Influences Grain Yields in Grasses

Field of Setaria viridis growing in western Nebraska. In the current issue of Nature Plants Huang and colleagues use Setaria viridis to identify the inflorescence mutant, sparse panicle 1. A mutation in the maize ortholog conditions a very similar panicle defect, demonstrating the utility of S. viridis for gene discovery in the panicoids. (Pu Huang)Genetic screen identifies mutations that impact green foxtail panicle formation. The Science Through deep sequencing of the model grass green foxtail (Setaria viridis), researchers pinpointed a gene critical for the development of flowers that give rise to the grain. Using this information, a homologous gene in maize was identified as playing a similar role highlighting… [Read More]

April 3, 2017

Speciation Driven by Alleles Adapted to Local Conditions

For this study, researchers planted a test population in a mountain-top meadow near the Lost Trail Pass ski resort in the mountains of Montana. To water these transplants, they lugged nine empty garbage cans up the mountain and filled them with snow to water the plants throughout the summer. (Tom Mitchell-Olds)Genome sequence analysis provides first evidence that linked, locally adapted alleles exist first, and subsequently are captured within a new, selectively-favored inversion. The Science A team of scientists investigated the pathway by which chromosomal inversions – in which a segment of the chromosome was removed, flipped and then re-inserted – contribute to speciation in nature…. [Read More]

March 29, 2017

Grasses: The Secrets behind Their Stomatal Success

John Vogel with Brachypodium plants at the DOE JGI. (Roy Kaltschmidt, Berkeley Lab)Finding a grass gene impacting stomatal morphology underscores importance of developing mutant gene index. The Science The evolution of adjustable pores, stomata, allows plants to control the amount of CO2 that enters the plant and water that escapes by modifying the stomatal pore size. Plants have evolved two kidney shaped guard cells that swell to… [Read More]

March 14, 2017

New Software Tools Streamline DNA Sequence Design-and-Build Process

DOE JGI BOOST logoImprove on existing biological computer-aided design and manufacture (bioCAD/CAM) tools The Science Synthetic DNA allows scientists to expand the breadth and depth of their genomic research. In this study researchers from the U.S. Department of Energy Joint Genome Institute (JGI) have developed a suite of build-optimization software tools (BOOST) to streamline the design-build transition in… [Read More]

March 6, 2017

Nitrogen Uptake Between Fungi and Orchids

Researchers investigated Tulasnella calospora as both a free-living mycelium and in symbiosis with the photosynthetic orchid long-lipped serapias. (Ziegler175, Wikimedia Commons, CC BY-SA 3.0)Fungal and plant gene expression provides clues to nitrogen pathways. The Science Orchids are an example of an experimentally tractable plant that is highly dependent on its relationship with its mycorrhizal fungal partners for nutrient supply. In this recent study, researchers for the first time identified some genetic determinants potentially involved in nitrogen uptake and… [Read More]

February 6, 2017

Metagenomics Leads to New CRISPR-Cas Systems

The newly discovered CRISPR-CasY system was found in bacteria from deep underground at Crystal Geyser in California. (Jill Banfield, UC Berkeley)First CRISPR-Cas9 system in archaea discovered The Science Through metagenomic data generated by the DOE Joint Genome Institute, researchers analyzed 155 million protein coding genes from uncultivated microbial communities, leading to the discovery of the first CRISPR-Cas9 protein in the archaeal domain, and of two previously unknown simple bacterial CRISPR-Cas systems. The Impact Microbes play… [Read More]

December 19, 2016

Of Mutualism and Lipid Metabolism in Fungi

Hyphae and asexual sporangia of Rhizopus microsporus (photo by Stephen Mondo). Inset shows a hypha with YFP-labeled Burkholderia endobacteria in green and DAPI-stained fungal nuclei in blue; scale bar, 5 um (photo by Olga Lastovetsky)Novel mechanism in bacterial-fungal symbiosis could have biodiesel production applications The Science To answer the challenge of producing renewable, sustainable alternative fuels, researchers aren’t just looking at developing candidate bioenergy crops but are also reviewing other natural sources of energy-dense oils such as fungi. To learn more about how bacteria interact with fungi in a… [Read More]

December 9, 2016

DOE JGI Database of DNA viruses and retroviruses debuts on IMG platform

This graphic depicts the geographic distribution of GOLD biosamples and organisms. Organism location of isolation is marked in pink while Biosample location of collection is denoted with blue dots. Updates to the Genomes OnLine Database (GOLD) are reported in the upcoming Database issue of Nucleic Acids Research. (Image from Supratim Mukherjee et al. Nucl. Acids Res. 2016;nar.gkw992)The Science In a series of four articles published in the Database issue of the Nucleic Acids Research journal, DOE JGI researchers report on the latest updates to several publicly accessible databases and computational tools that benefit the global community of microbial researchers. One report focuses on a new database dedicated global viral diversity. [Read More]
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