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June 11, 2015

Charting Short-Term Results of Wetlands Restoration

After a day collecting samples from Twitchell Island, located in the Sacramento-San Joaquin Delta of California, USGS scientist and study co-author Lisamarie Windham-Myers (left), study senior author Susannah Tringe (center), and study first author Shaomei He (right) go over their preliminary findings. (Image by David Gilbert, DOE JGI)In mBio, JGI researchers reported on microbial community composition and carbon emissions patterns from restored wetlands. [Read More]

May 22, 2015

The Most Complete Functional Map of an Entire Enzyme Family

Overlay of SacteLam55A onto PcLam55A (Image from Bianchetti CM et al, J Biol Chem. 2015 May 8. doi: 10.1074/jbc.M114.623579. )DOE-funded researchers develop a new process for annotating cellulose-degrading enzymes. The Science: Researchers at two Department of Energy-funded Scientific User Facilities collaborated with one of three Bioenergy Research Centers to develop and analyze high-resolution crystal structures of an enzyme from the cellulose-degrading GH55 family. They then went further and were able to apply a variety… [Read More]

May 1, 2015

Understanding the [Non-Coding] Spaces Between

The golden-brown surface mats that indicate a Trichodesmium erythaeum bloom have led to them being called “sea sawdust.” (Image by FWC Fish and Wildlife Research Institute via Flickr CC BY-NC-ND 2.0)DOE JGI researchers sequenced and analyzed a cyanobacterium with known nitrogen-fixing capabilities. The Science: Researchers sequenced and analyzed the uncommonly large genome of a nitrogen-fixing cyanobacterial strain, comparing it to the more than 150 extant cyanobacterial genomes. The Impact: Unlike many of the existing cyanobacterial genome sequences, the genome of Trichodesmium erythaeum IMS 101 is… [Read More]

April 15, 2015

Big Plant Genomes: Formerly Intractable, No Longer Insurmountable

wheat image by Jordi Paya Canal via Flickr, CC-BY-SA-2.0DOE JGI researchers have developed an assembly and mapping strategy for any species, including large and complex genomes. The Science: Through a combination of high-throughput sequencing, high performance computing, and genetic mapping, DOE JGI researchers have derived a sequence assembly for the highly repetitive plant genome of bread wheat (Triticum aestivum). The Impact: The researchers… [Read More]

March 27, 2015

Targeted Sorting of Microbial Cells

DOE JGI is enabling collaborators to develop a universally applicable technique for studying microbial metabolic activities. The Science: A team led by University of Vienna researchers has developed a way to identify and sort single microbial cells through a probe-independent process that uses heavy water (laced with deuterium) which is then incorporated into mostly lipids… [Read More]

March 24, 2015

Identifying causes of poplar canker

Cankers caused by the fungal tree pathogen M. populorum on poplar stems. (T.H. Filer Jr., USDA, Bugwood.org CC BY-NC-3.0)Researchers compared two fungal tree pathogens to find out how one of them has gained the capability to significantly damage hybrid poplar plantations. [Read More]

February 3, 2015

Complete Genomes from Single Cells Still Elusive

Clingenpeel et al image from Frontiers in Microbiology articleDOE JGI researchers describe the current challenges in single-cell genomics. The Science: DOE JGI researchers review the status of single-cell genomics, and how close scientists are to being able to reconstruct an individual cell’s genome. The Impact: As an alternative method of studying microbial communities, single-cell genomics allows researchers to link function to phylogeny without… [Read More]

January 20, 2015

Enhancing Microbial Pathways for Biofuel Production

refueling a car with biodiesel. image from Flickr CC United Soybean BoardDOE JGI, JBEI researchers collaborate on improving terpene production in E. coli. Science: Researchers from the DOE JGI and the Joint Bioenergy Institute identified genes in an E. coli microbial metabolism pathway that could improve the production of terpenes. The Impact: Terpenes are high-energy compounds produced in microbes and plants that could be used for… [Read More]

December 22, 2014

How a White Rot Tackles Freshly-Cut Wood

Scanning electron microscopy (SEM) image of pine that was substantially eroded by the white rot P. gigantea. Bar = 40 µm. (Image from Hori et al. PloS Genet. doi:10.1371/journal.pgen.1004759.g002)Unlike many other white rot fungi, P. gigantea prefers to colonize freshly-harvested wood. The Science: Researchers sequenced and analyzed the white rot fungus Phlebiopsis gigantea, which can break down fresh-cut conifer sapwood. They also sequenced and analyzed the set of P. gigantea’s secreted proteins (secretome) and the set of all of its RNA molecules (transcriptome)…. [Read More]

November 26, 2014

Eucalyptus grandis Genome 2.0—Community Resource

eucalyptus plantation courtesy of the University of PretoriaGenetic maps improve the reference genome assembly of the candidate bioenergy feedstock The Science: A team of French researchers developed an array that allowed them to produce high-resolution genetic maps of two eucalyptus species that they then compared to the reference genome of eucalyptus produced by a team including DOE JGI researchers. The Impact: The… [Read More]
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