The analysis of gene expression allows scientists to generate hypotheses important for the understanding of gene function. Proteins that participate in similar functions, or form larger protein complexes, are often encoded by genes that share similar expression profiles. This type of analysis has been limited to “steady-state” mRNA levels, that is the combined output of transcription and mRNA degradation. The proposal aims to measure these two components: how many mRNA molecules are produced at any given time during the day, and how stable are these RNA molecules over time.
Proposer: Patrice Salome, University of California, Los Angeles
Proposal: Control of mRNA half-life Across the Diurnal Cycle in the Model Alga Chlamydomonas