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Single-cell isotope tracing reveals functional guilds of bacteria associated with the diatom Phaeodactylum tricornutum

Bacterial remineralization of algal organic matter fuels algal growth but is rarely quantified. Consequently, we cannot currently predict whether some bacterial taxa may provide more remineralized nutrients to algae than others. Here, we quantified bacterial incorporation of algal-derived complex dissolved organic carbon and nitrogen and algal incorporation of remineralized carbon and nitrogen in fifteen bacterial… [Read More]

Lower viral evolutionary pressure under stable versus fluctuating conditions in subzero Arctic brines

BackgroundClimate change threatens Earth’s ice-based ecosystems which currently offer archives and eco-evolutionary experiments in the extreme. Arctic cryopeg brine (marine-derived, within permafrost) and sea ice brine, similar in subzero temperature and high salinity but different in temporal stability, are inhabited by microbes adapted to these extreme conditions. However, little is known about their viruses (community… [Read More]

A telomere-to-telomere genome assembly resource of Bipolaris sorokiniana, the fungal pathogen causing spot blotch and common root rot diseases in barley and wheat

Bipolaris sorokiniana (=Cochliobolus sativus) is a fungal pathogen that causes spot blotch, common root rot, and kernel blight in barley and wheat. Four pathotypes (0, 1, 2, 7) of the fungus were previously identified based on their virulence on three barley differential lines (Bowman, ND 5883 and NDB 112). Although several genome assemblies have been… [Read More]

Transcriptomic and genomic effects of gamma-radiation exposure on strains of the black yeast Exophiala dermatitidis evolved to display increased ionizing radiation resistance

Melanized fungi thrive in extreme environments, including those with high levels of ionizing radiation. To understand the role that melanin may play in ionizing radiation resistance, we previously performed an adaptive laboratory evolution experiment in which we used melanized and non-melanized strains of the yeast Exophiala dermatitidis to develop evolved lines that exhibit increased ionizing… [Read More]

BinaRena: a dedicated interactive platform for human-guided exploration and binning of metagenomes

BackgroundExploring metagenomic contigs and “binning” them into metagenome-assembled genomes (MAGs) are essential for the delineation of functional and evolutionary guilds within microbial communities. Despite the advances in automated binning algorithms, their capabilities in recovering MAGs with accuracy and biological relevance are so far limited. Researchers often find that human involvement is necessary to achieve representative… [Read More]

Secreted Effector Proteins of the Poplar Leaf Spot and Stem Canker Pathogen Sphaerulina musiva Manipulate Plant Immunity and Contribute to Virulence in Diverse Ways.

Fungal effectors play critical roles in manipulating plant immune responses and promoting colonization. Sphaerulina musiva is a heterothallic ascomycete fungus that causes Septoria leaf spot and stem canker disease in Populus plantations. This disease can result in premature defoliation, branch and stem breakage, increased mortality, and plantation failure. However, little is known about the interaction… [Read More]

Custom tuning of Rieske oxygenase reactivity

Rieske oxygenases use a Rieske-type [2Fe-2S] cluster and a mononuclear iron center to initiate a range of chemical transformations. However, few details exist regarding how this catalytic scaffold can be predictively tuned to catalyze divergent reactions. Therefore, in this work, using a combination of structural analyses, as well as substrate and rational protein-based engineering campaigns,… [Read More]

Solvent-Producing Clostridia Revisited

The review provides an overview of the current status of the solvent-producing clostridia. The origin and development of industrial clostridial species, as well as the history of the industrial Acetone Butanol Ethanol fermentation process, is reexamined, and the recent resurgence of interest in the production of biobutanol is reviewed. Over 300 fully sequenced genomes for… [Read More]